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Conceived and designed the experiments: JAD. Brycon spp. Coastal rivers in southeastern Brazil are characterized by the presence of relatively few freshwater fish species and high endemism of this fauna. The objective of this study was to examine whether Brycon spp. All the species showed a diploid number of 50 chromosomes, a conserved number within the subfamily Bryconinae. All of them had nucleolar organizer regions in the first pair of subtelocentric chromosomes and no equilocal distribution of heterochromatin in the first pair of chromosomes of the karyotype.
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